donkey

Assessment of the efficacy of the anthelmintic moxidectin against cyathostomins at The Donkey Sanctuary: analysis of new and historical data using novel statistical methods

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Applicant(s)
Methodology

Statistical analysis of retrospective data. Phase one involves transfer of data to the lead investigator for analysis using Marcov chain Monte Carlo (MCMC) statistical methods. Phase two involves combined analysis of all FECRT data collected over the three sampling times to identify i) temporal trends in the development of resistance, ii) evidence for differential rates of development of resistance, and iii) evidence for particular groups of animals for which the rate of development of resistance seems to be accelerated compared to the other groups. Phase three of the project will involve communication of the findings to the research and clinical teams at The Donkey Sanctuary, and subsequent discussions regarding the implications of these findings for routine endoparasite control.

Aims

The overall aim of the project is to analyse the patterns of changing drug resistance to the clinically important anthelmintic Moxidectin within the small strongyle (cyathastomin) parasite population at The Donkey Sanctuary. Overall control of the endoparasite population is a complex issue so the clinical recommendations generated as part of this proposed project will be given within the context of a much larger, previously completed collaborative research project. An important part of this project is to discuss findings with the clinical and research teams at The Donkey Sanctuary in the context of recommendations for both routine annual dosing of all animals and treatment of animals with clinical disease and/or welfare compromise due to endoparasites.

Objectives

1) To examine faecal egg count reduction test (FECRT) data gathered as part of routine annual anthelmintic use at The Donkey Sanctuary autumn/winter 2018/2019, in order to assess the current efficacy of Moxidectin using state of the art statistical methods. 2) To use bespoke statistical methods to analyse the most recent FECRT along with similar data during autumn/winter 2010 and 2013 to identify trends in the pattern of resistance development, including associations between accelerated rates of resistance development and particular management patterns. 3) To interpret the findings of this project in relation to those of previous projects, and to contribute to the established recommendations for best practice to minimise the rate of development of drug resistance at The Donkey Sanctuary.

Investigation of asinine pulmonary fibrosis

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Methodology

Non-invasive clinical examination, case/control examination of post mortem material, case review of clinical and pathology records. Whole lungs were collected from 32 aged donkeys (19 APF-like on gross examination and 13 controls) at routine necropsy from 2 UK Donkey Sanctuaries between June 2009 and January 2013. 18 whole inflated ex vivo lungs (11 APF, 7 controls) were imaged with high resolution computed tomography (HRCT) while the remainder were sectioned and photographed digitally. Tissue samples were collected from all lungsinto 10% buffered formalin and processed into paraffin blocks for histopathological evaluation . HRCT images and histology sections were objectively reviewed and categorised as, 'consistent with' or 'inconsistent with' characteristics of pleuroparenchymal fibroelastosis (PPFE). Tissue samples from 8 lungs were also processed for DNA extraction and PCR. Lung sections with granulomatomous inflammation were analysed for fungal hyphae, and Mycobacteria. X-ray diffraction was used to identify both fibrous and non-fibrous dust content.

Aims
  1. To characterise Asinine Pulmonary Fibrosis (APF) on a gross, histopathological and molecular level.
  2. To perform an epidemiological study, encompassing case histories, environmental management, and potential pathogen and dust exposure.
  3. Evaluating non-invasive clinical techniques including detailed clinical examination, pulse oximetry and thoracic ultrasound as early diagnostic and disease monitoring tools.
Objectives

To examine ex vivo lung tissue from a small cohort of aged donkeys with a high prevalence (35%) of fibrosing interstitial lung disease named Asinine Pulmonary Fibrosis.

Results

There was no statistically significant difference between the age of APF and control donkeys. Ten of 19 APF cases were categorised as being consistent with PPFE according to the defined histological and imaging criteria sharing key features with human PPFE. As donkeys are quadruped, the dorsal lung equates to the human upper zone. All 10 PPFE-like lungs had grossly visible visceral pleural fibrosis on the dorsal/costal surface, with no involvement of the parietal pleura. Histologically, all 10 PPFE-like lungs had marked pleural and subpleural fibrosis, predominantly within the upper lung zone, with accompanying intra-alveolar fibrosis and elastosis. It is possible that some of the DPF lungs classified as 'inconsistent' with PPFE may represent an earlier stage of PPFE-like disease. All control cases were classified as inconsistent with PPFE on histology and HRCT. Asinine herpes virus was ubiquitously expressed within both control and APF lung tissue. No other etiologic agents were identified. X-ray diffraction analysis revealed dust contents and small numbers of fibrous and non-fibrous particles.

Conclusions

This study showed that over 50% of donkeys with DPF shared key pathological and imaging characteristics of human PPFE, a rare and usually idiopathic interstitial pneumonia. Further study of APF may yield valuable information to help elucidate the etiopathogenesis of this emerging human disease; in particular how recurrent lower respiratory tract infections may contribute to the pathogenesis of PPFE.

Development and quantitative validation of improved sustainable donkey parasite control programmes (extension): creation and comprehensive validation of a general monitoring and control system for donkey endoparasites

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Methodology

Refinement of existing statistical modelling. Development of bespoke software solution for decision support and monitoring. Case comparison study to test new software.

Aims

To work towards 'best practice' for comprehensive donkey parasite control, continuing the aims of an existing collaborative studentship focussed on cyathostomin management and expanding the remit to include all endoparasites of welfare concern to animals at The Donkey Sanctuary. The ultimate goal is incorporation of the information available from all potentially useful sources into the same comprehensive framework, producing an evidence based targeted anthelmintic dosing programme to control pasture infectivity, as well as monitoring other indicators of individual animal welfare compromise from veterinary records and signs of clinical parasitism from other relevant endoparasites at post mortem. This approach will continue to incorporate methods of environmental suppression of parasite transmission, which is a sustainable and effective way of controlling many parasites of grazing animals.

Objectives

In addition to the previous project: 1. To extend the remit of the decision support system being developed to account for all other pathogenic endoparasites that compromise animal welfare at The Donkey Sanctuary. 2. To use climatological information from an automated weather station to increase the accuracy of the strongyle targeted dosing system and to predict high risk periods for liver fluke. 3. To incorporate information from the individual animal veterinary records into the targeted dosing system, so that any individual animal history of clinical signs that are found to be consistent with parasitism can be accounted for in making dosing decisions for the group. 4. To monitor information on post-mortem findings and relate this to the clinical findings, FWEC and other data available for the surviving members of the same group to provide an early warning system for a wide range of emerging parasite issues 5. To develop an alternative retrieval interface to the existing SQL database, to enhance the usefulness of this data and efficiency of FWEC recording at The Donkey Sanctuary. 6. To deliver a bespoke software solution delivering the database access, information retrieval and decision support system developed during the studentship along with automatically generated targeted dosing recommendations and feedback of early warning indicators for disease to the veterinary team.

Keywords

Protection from the elements - part one: a comparative study of shelter use, hair density and heat loss in donkeys, horses and mules

Status
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Collaborator(s)
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Methodology

Observational data and quantitative measurements. Modelling using Generalised Linear Mixed Models (GLMM) to compare variables.

  1. A study of shelter seeking behaviour

Observations made 1-2 x per week for each animal for behaviour (location, activity, insect harassment and time of day) and for weather (temperature, precipitation, wind speed, luminous flux and shelter conditions)

  1. A study of hair density

5cm squared Small area of neck hair is clipped four times a year, dried and weighed. Hair strand thickness and length is also assessed.

  1. A study of heat loss

Comparable data exists for horses. Scientific measurement of actual heat loss from different body parts. Images taken in different ambient temperatures of dry and wet animals.

Aims
  1. To assess the protection donkeys (and mules) require when kept in the UK (and compare this with horses).
  2. To provide scientific evidence that can inform government, equine charity and equine industry guidelines.
  3. To improve equid welfare.
Results
  1. Shelter seeking study. Donkeys spend more time indoors than horses. Donkeys seek shelter more than horses when it rains. Donkeys are more affected by changes in temperature in UK than horses. Donkeys stay inside more when the temperature is below 10. Perhaps some evidence horses start to seek shelter more when temperature is above 20. Although there were no observations in high winds, donkeys may be more affected by changes in wind in the UK than horses. Donkeys may seek shelter in moderate winds. Wind effects are likely to interact with insect density. Horses may be more affected by insect density than donkeys. Other factors may influence donkey shelter use more than insects. Relative (rather than absolute) insect density will affect shelter use.
  2. Hair density study. In winter donkeys and mules have thinner hair than horses. In spring donkeys have thinner hair than horses. There was no significant change in hair weight in donkeys. Horses grow a winter coat. Mules grow a winter coat but don’t seem to lose it as quickly as horses. Hair weight and length only correlate in spring in horses and donkeys.
  3. Heat loss study. Data collection was halted, it was decided not to proceed with this part of the project.
Conclusions

It was found that donkeys spend comparatively more time in shelters than horses do. Donkeys were seen to seek shelter more readily when raining or cold, whereas horses sought shelter when temperatures rose above 20°C. Donkeys showed an increase in shelter use when moderate wind speeds were recorded, where-as horses were largely happy to remain outside. When insect harassment outside increased, donkeys seemed more tolerant compared to horses. Compared to horses, donkeys don’t grow significant winter coats and have thinner and shorter hair. Mules were found to be ‘in-between’ when comparing horses and donkeys hair coat properties.

These differences in results seem to reflect the donkeys’ and horses’ evolutionary background. Donkeys are more tolerant of higher temperatures than horses, but not as tolerant of cooler, wetter weather. This, coupled with the donkey’s thinner coat, suggests that the shelter requirements for the two species are different, with donkeys requiring additional protection from the elements in temperate climates.

 

Understanding the socio-economic impact of donkeys

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Methodology

In depth interviews, participatory rural appraisal (PRA) exercises, case sample evaluations

Aims
  1. Use of in-depth interviews to provide an overview of the different types of social and economic impacts that working donkeys can have in communities.
  2. Development of a series of Participatory Rural Appraisal (PRA) exercises to investigate and partially quantify the socio-economic impact of working donkeys.
  3. To complete case study evaluations of the socio-economic impact of working donkeys in areas of Ethiopia, to both test and validate the developed approach and to provide new data about the role of working donkeys and mules in the lives of people living in Ethiopia.
  4. To conduct animal-based welfare assessments to complement the socio-economic data gathered in case study areas to investigate relationships between donkey owners' perceptions of the value of their animals and the animal's welfare.
Results

1) Areas of life affected by donkey ownership identified by use of in depth interviews:

  • economic impact
  • provision of care for donkeys
  • empowerment
  • gender
  • social status
  • affect (emotions expressed about owning/working with donkeys)
  • vulnerability & resilience

2) A series of Participatory Rural Appraisal (PRA) exercises or tools were developed to explore the socioeconomic impact of donkeys.

Nine different tools were developed (including:

Value Web

Dream and Discover

Gender Myths

Empowerment and Status

Matrix of Species

Donkey Expenditure

100 Seeds Seasonal Calendar

Livelihoods Pair-wise Matrix

Who is Your Donkey?

3) Twelve workshops were conducted (two per case study, divided into male and female workshops) in six different communities (three urban and three rural).

a) Donkeys are direct generators of income in the following ways:

  • Pulling carts to transport goods for sale
  • Carrying construction materials in pack saddles
  • Pulling rubbish carts
  • Being rented out to non-donkey owners
  • Occasional use for ploughing and planting agricultural fields
  • Breeding and selling offspring

 

b) Donkeys are also a source of indirect income generation in the following ways:

  • Transporting goods for use (such as firewood) or for bartering
  • Transporting goods to support another form of income (such as feed for cattle or pulling sick animals to a clinic by cart)

c) After meeting their basic needs, people who use donkeys could save money and contribute to community saving organisations (which act as informal banks and insurance programs).

d) Possible non-financial gains from donkey owning include increase in social status, empowerment and sense of pride, contributing to local society, involvement in decision making, and relief from carrying physical burdens and associated health implications.

4) Welfare assessment overview

  • 161 donkeys were sampled (111 stallions and 50 mares)
  • 53% between 5 and 15 years old
  • 64% had an apathetic demeanour
  • 93% had skin lesions (most commonly on hindquarters and tail base)
  • 60% had gait abnormalities
  • 91% had abnormal hoof shapes
  • 64% had signs of limb tethering
  • 54% were thin (body condition score 1.5-2)
  • 51% showed signs of mild to moderate heat stress
  • The majority of working donkeys assessed had two or more welfare issues
Conclusions

The results from the study of the socio-economic impacts of working donkeys in Ethiopia reveal that donkeys are major contributors to people’s lives no matter what context they are living and working in; for their owners, donkeys are the difference between destitution and modest survival. Sadly, the drudgery performed by donkeys is often a reflection of the lives of their owners; their hardships are shared. Donkeys become pathways out of poverty for the poor in rural and urban areas by providing access to opportunities through transportation for income generation, to attend schools, markets, and health clinics. The use of donkeys allows people to earn and save money and increase their ability to contribute to community savings and loans schemes.

Donkey owners expressed acute dependence on their donkeys and articulated feelings of love and friendship, even respect. Donkeys are understood to be animals that make people’s lives easier and act as mutual friends and life supports. Owners describe the importance and value that donkeys bring to their own lives and to their communities, however, although donkeys are undervalued and underappreciated by broader Ethiopian society. Donkeys are seen as ‘low-class’ animals of the working poor and the immense benefits they bring to those who own them remain largely invisible. Furthermore, donkeys’ social position within society is viewed as similar to that of women and, sadly, both are said to be treated equally—being beaten, carrying large loads on their backs and being submissive to men.

Where donkeys are in regular usage they are seen as important animals, friends or even part of the family, and people would find it difficult to make ends meet or provide for their families if they did not own a donkey. Humans rely on donkey transport to health services, to create their livelihoods, to relieve them of physical burdens and prevent them from slipping into extreme poverty. Donkeys rely on humans for feed, veterinary care, shelter, safety, and rest. Thus, donkey and human health are interconnected. However, donkey owners struggle to provide care to their families and livestock; provision of care to donkeys features low down on owners’ priority lists. As a result donkey welfare suffers. Donkey welfare assessment results show that the majority of donkeys surveyed suffered from gait abnormalities, abnormal hoof shapes, a high prevalence of body lesions, poor body condition scores, and displayed behaviours potentially indicative of negative emotional states.

Despite the donkeys important contributions to many of Ethiopia’s poor they remain absent from government animal health and welfare policies, they are overlooked by development goals and are marginalised by wider society. Thus, an exploration of the human dimensions of donkey wellbeing is crucial for understanding and properly valuing the donkeys’ impact in developing countries.

Reframing benefits of equid assisted activities: An analysis of engagement between autistic children and donkeys

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Research award
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Methodology

Capturing interspecies interactions demanded a methodology that was flexible enough to include children and donkeys as equal participants. Many of the autistic children who took part in this research were non-verbal, therefore the methods utilised for exploring their inner lives and affective states were compatible with those often used to study non-human animals.  

What is lost with some quantitative research methods is the temporal sequence of events during relationship formation which was seen as critical in donkey assisted therapy (DAT) sessions. Capturing individual stories by using multispecies ethnography complemented the quantitative data and offered examples of the context and tangential variables surrounding interactions that would be impossible to control for in a quantitative repeated measures design.  

Aims
  1. To explore if The Donkey Sanctuary donkey assisted therapy program continued to be beneficial for both children and donkeys as the founder Elisabeth Svendsen had intended when she set it up in the mid 1970’s. 

  2. To focus on exploring the child and donkey relationship by viewing both as active participants (much of previous body of work focussed only on the human’s responses). 

  3. To rigorously explore the engagement between child and donkey, with a view to informing practice going forwards. 

Objectives
  1. To develop a Quality of Engagement tool 

  1. To investigate and answer the following questions: a) how does one member of a child-donkey dyad affect the other in equid assisted activities (EAA), and b) do participants act differently with members of their own species as opposed to the other?  

Results

The research confirmed that interspecies engagement can be measured equally for both species and the results can be used as an indicator for welfare and consent of non-verbal participants. It did not, however, show that such engagement reflected outcome benefits. ‘Benefits’ are subjective and dependent on how and of whom the question is asked. Other EAA research has indicated that there are positive ‘benefits’ from EAA, yet they have not included measures of engagement their participants actually experienced. Without a clear indication of engagement between partners, it is not possible to attribute these benefits solely to the EAA. The undefined yet very special ‘thing’ that is so attractive about donkeys and horses should be observed as a concrete variable, such as their emotional state and motivation to engage for the purpose of EAA research. 

The relationship between autistic children and donkeys relies on the uniqueness of each character on any given day. Autism is not a homogeneous description of a person, and whether it is considered a diversity or a diagnosis, it still translates to each person being unique. Both humans and donkeys are individual characters made up by their culture, family group norms, peer norms, genetic makeup, individual histories, and individual personalities. How they will relate to one another is very hard to predict and therefore requires constant monitoring for both welfare and consent. 

Autistic children and donkeys were shown to be a compatible coupling because some of their traits were similar and complemented their interactions. Both cohorts required a calm environment to be able to focus, smooth and fluid movements from others around them and as much time as they needed to process information. EAA is not a lifesaving medical intervention therefore it should only be considered as a practice for those children who show a willingness towards interactions with other species. Equally, some donkeys may find certain individuals or environments distressing and this should be monitored and respected.  

Conclusions

The findings of this research showed that both human and donkey partners were affected by the other and many of those interactions were considered positive, but not all. The donkey participants in this research significantly modified their behaviour to accommodate the children. Donkey behaviour was different when they were with other donkeys showing their awareness of the vulnerabilities of children in EAA sessions. Children were also affected by the other partner in the dyad. Some were generally more aversive towards donkeys than they were to other children which could be attributed to species communication differences however, some children were able to relax more when with donkeys than other children.  

The ethnographic stories highlighted the differences between human-donkey dyads and just how difficult it is to direct individuals towards meaningful relationships within a given environment or timeframe. This is the both the crux and the mystery of EAA. It is the understanding of who the other is and the resulting quality of engagement that dictates the interpersonal experiences within sessions.  

Use of the Modified AWIN Welfare Assessment Protocol combined with a novel computer-based Animal Management System as a tool for managing herd health in donkeys

Presentation date

The Donkey Sanctuary is a global welfare charity whose mission is "To transform the quality of life for donkeys, mules and people worldwide". In the UK, The Donkey Sanctuary cares for over 2000 donkeys on a number of farms varying in size from 250-580 animals. The farms aim to rehome approximately 10% of their herd annually to guardian (private) homes or donkey assisted therapy centres. The farms also provide a show case for our work to visiting public and professionals. Welfare of the donkeys on the farms is critical to the credibility of The Donkey Sanctuary.

The veterinary team monitors the herd health to maintain high welfare standards. Traditional input consisted of weekly visits, annual vaccination, dental treatment, parasite control, and a reactive approach to illness. Since the introduction of The Donkey Sanctuary Animal Management System and Animal welfare assessment protocols(AWIN), the team have been able to use evidence -based criteria (EBC) to assess farm herd health.

Methods

Every four months 1) the AMS data base is interrogated supplying information on relevant queries including Body Condition Score, Lameness, Colic, Hyperlipaemia, Sarcoids, Infectious disease, and Mortality rate, 2) an on farm welfare assessment is performed following the stage 1 AWIN (Animal Welfare Indicators) protocol for donkeys.

Resource based and animal- based indicators are assessed on a randomly selected 10% of the herd by a team including vet, farm manager and grooms. Different animals are selected at each visit by using the farm named list of donkeys.

Results

Results are recorded via excel, graphical representation and written documentation. The results can be sub-divided in many ways depending upon clinical need.

Discussion

Using EBC and AWIN provides the vet team with tools to pro-actively monitor donkey health, refine management practices, re-direct budgets and track progress. Welfare can be bench marked and improvements aimed for. Monitoring / recording welfare data allows The Donkey Sanctuary to be compliant with national legislation. Although a number of welfare assessment tools are available AWIN is validated and straightforward to use. The poster illustrates 4 quarters of data presented graphically.

EARS - Equid assessment research & scoping tool: a new approach to analyse, understand and respond to equid welfare problems worldwide

Presentation date

The capacity for decision-making and intervention in any project related to equid welfare should be based on a knowledge of the real issues affecting these animals worldwide, regardless of the tasks performed. This approach is even more important when new emerging economic activities around equids - e.g. donkey skin trade - are particularly challenging in terms of animal welfare.

The Equid Assessment Research & Scoping (EARS) Tool is a questionnaire developed by The Donkey Sanctuary, in collaboration with World Horse Welfare, with the main purpose to provide reliable information about the general health and welfare of equids worldwide. EARS is primarily designed to obtain individual information about an equid and its surrounding environment, or from a group of equids in similar conditions, through cumulative repetition. It is organized into 18 indicators, each one divided in to different categories, and each category with a specific set of questions. The EARS Tool allows the development of different protocols, by choosing the correct set of questions that best fit the inherent needs.

Data collection and preliminary analysis of data in a simple, fast and effective way are also a central aspect of EARS, by using open source software (OSS) throughout the data collection cycle: Open Data Kit Collect to log information in the field; R to parse, analyze and filter this information; and R Shiny to disseminate the results through dashboards. These OSS products allow off-line data collection with initial overview results presented as soon as the user uploads their surveys when back online.

This new tool contributes to a better understanding of the underlying reasons behind poor equid welfare, and allows the design of evidence-based strategies that not only identify and tackle the real causes of problems, but also allow the organizations to measure the impact of decisions taken over time.

Hide nor hair – the illicit trade in donkey hides is a threat to wild asses

Presentation date

The global donkey population is estimated at 44 million and is largely associated with economically developing nations where donkeys are used as working animals. Donkeys play a central and critical role in supporting the livelihoods of millions of people accross the world, providing support for farming, enabling access to resources, and in food production. Global demand for diverse products of donkey origin has escalated rapidly, with a particular interest in the premium products resulting from donkey skins.

Ejiao is a traditional Chinese medicine product which is based upon extracts of donkey gelatin from donkey skins, mixed with herbs and other ingredients to form a gelatinous bar, which is marketed as a miracle cure for multiple health problems. Since 2010,  consumer demand for ejiao has increased rapidly, and subsequently, so has the demand for donkey skins. The Donkey Sanctuary estimate that a minimum of 1.8 million donkey skins are being traded per year, but this may be a gross underestimate. The increasing wealth and diaspora of the Chinese middle classes, alongside the apparent credibility of ejiao products, appears to have created such a high level of demand for donkey skins that global supply is struggling to keep up, leading to high prices and widespread claims of fraud. Such high levels of demand by the Chinese market are fuelling global reports of donkey theft and a sudden increase in the purchase price of donkeys. Products of donkey origin are so highly sought after that ejiao can sell for up to USD $500/kg.

The alarmingly high demand for donkey skins, and high prices that a donkey skin can fetch, positions donkey skin in a similar position as ivory or rhino horn. There is a complete lack of regulation over the utilisation of donkeys for the skin trade to fuel ejiao production, and new slaughter houses are opening at rapid rates to keep up with demand. Consequently, there has been a sharp rise in donkey thefts. As well as being unsustainable and harmful to rural livelihoods, this illicit trade could have devastating effects on populations of wild asses. Donkey skin is highly valuable, yet increasingly  scarce, making it feasible that traders will start targeting wild asses. The Afrcan Wild Ass Equus africanus is Critically Endangered, with fewer than 200 mature indivduals remaining in the wild; unfortunately, the species occupies an area of Africa where the trade in donkey skins is high, exposing it to risk of being targeted. There is also some indication that populations of Asiatic Wild Ass Equus hemionus could also come under threat, given their proximity to China and surrounding socioeconomic climate.

The international trade in donkey skins has emerged rapidly and fiercely, and is grossly unsustainable. The trade has the significant potential to eradicate populations of  donkeys across Africa, and poses a serious threat to the conservation and survival of African Wild Ass populations. In this presentation I raise and highlight these issues, and open up discussion for how this new threat may be mitigated for the conservation and management of wild equids.

Engagement behaviour of donkeys during equine assisted activities

Presentation date

There is a wealth of research citing the benefits of Equid Assisted Interventions (EAI's) but these are often anthropocentric and fail to include measures that capture donkey engagement behaviours during sessions. This observational bias, possibly quite common in EAI research, reinforces the assumption that animal activities are somehow enriching for the non-human animal thus welfare interests can be overlooked. By designing and implementing a unique Quality of Engagement Tool (QET), I was able to capture engagement behaviours of both donkeys and autistic children during interaction sessions and identify potential enrichment or welfare measures for both species participants.

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